All algorithms

3D classification

Stage

2

of 8

Included on all plans

Automated Class3D Convergence

3D classification that stops as soon upon convergence, instead of running a fixed number of rounds that no longer change the result.

Up to 30%

Of 3D classification runtime saved

Automatic

No iteration count to guess

What it does

3D classification runs for a fixed number of iterations that you choose before you know how the data will behave. Set it too low and the classes have not settled. Set it high to be safe, and the last stretch of the run changes almost nothing while still costing compute and time.

Automated Class3D Convergence removes the guess. The job stops as soon as the iterations converge, which can save up to 30% of runtime.

How it works

Each iteration of classification reassigns particles between classes and updates the class maps. Early on, many particles move and the maps change a lot. As the classification settles, each iteration changes less, until further rounds stop changing the outcome.

That flattening point is convergence. Stopping there keeps the result of a full run while dropping the redundant iterations. How much that saves depends on the dataset: it is largest on data that settles early, where a fixed iteration count does the most unnecessary work.

Orientation coverage clustered at one pole, and its effect on a density map

Per class resolution and stability plotted against the iteration number. High class assignment stability and plateaus in resolution are good indicators of convergence.

Where it sits

It applies to 3D classification, and its parameters are accessible in the 3D classification job setup page.

Classification is one of the longer-running steps in a workflow and is often repeated, so time saved here compounds across a project.

At a glance

Stage

3D classification

Runs

Prior 3D classification

Hardware

Cloud compute

Inputs

A reference map from the data

Outputs

Junk classes for use as decoys

Replaces

Availability

Included

Stop paying for iterations that no longer change the answer.

Frequently Asked Questions

If it's not covered here, please reach out to us.
…or start your free trial and see for yourself.

Is there a free trial?

Yes — when you sign up, you'll get instant access to a 30-day free trial with full access to the CryoCloud web application. You'll receive 20 hours of compute time and 2 TB of storage. No setup or infrastructure needed. After the trial, you can upgrade to a paid subscription or contact us for custom plans — no interruption to your data.

How can I start using CryoCloud?

Either go directly to out signup page or simply send us an email to hi@cryocloud.io so we can invite you to our application. You can find more information on how to get started here.

Do I need to install anything?

No - you really just need a (simple) computer, internet access and an internet browser. We took care of everything else. Read more on it here

How does your risk-free trial work?

If you are unsatisfied, you can cancel your plan within 30 days and we will refund you at 100%. You can deactivate the subscription from your account settings.

Frequently Asked Questions

If it's not covered here, please reach out to us.
…or start your free trial and see for yourself.

Is there a free trial?

Yes — when you sign up, you'll get instant access to a 30-day free trial with full access to the CryoCloud web application. You'll receive 20 hours of compute time and 2 TB of storage. No setup or infrastructure needed. After the trial, you can upgrade to a paid subscription or contact us for custom plans — no interruption to your data.

How can I start using CryoCloud?

Either go directly to out signup page or simply send us an email to hi@cryocloud.io so we can invite you to our application. You can find more information on how to get started here.

Do I need to install anything?

No - you really just need a (simple) computer, internet access and an internet browser. We took care of everything else. Read more on it here

How does your risk-free trial work?

If you are unsatisfied, you can cancel your plan within 30 days and we will refund you at 100%. You can deactivate the subscription from your account settings.

Frequently Asked Questions

If it's not covered here, please reach out to us.
…or start your free trial and see for yourself.

Is there a free trial?

Yes — when you sign up, you'll get instant access to a 30-day free trial with full access to the CryoCloud web application. You'll receive 20 hours of compute time and 2 TB of storage. No setup or infrastructure needed. After the trial, you can upgrade to a paid subscription or contact us for custom plans — no interruption to your data.

How can I start using CryoCloud?

Either go directly to out signup page or simply send us an email to hi@cryocloud.io so we can invite you to our application. You can find more information on how to get started here.

Do I need to install anything?

No - you really just need a (simple) computer, internet access and an internet browser. We took care of everything else. Read more on it here

How does your risk-free trial work?

If you are unsatisfied, you can cancel your plan within 30 days and we will refund you at 100%. You can deactivate the subscription from your account settings.

Ready to skip the queue?
Start analyzing your cryo-EM data today.

We would love hear about your cryo-EM goals and discuss how we can accelerate them.

  • No infrastructure needed

  • Streamlined onboarding & legal support

  • Highest security

Ready to skip the queue?
Start analyzing your cryo-EM data today.

We would love hear about your cryo-EM goals and discuss how we can accelerate them.

  • No infrastructure needed

  • Streamlined onboarding & legal support

  • Highest security

Ready to skip the queue?
Start analyzing your cryo-EM data today.

We would love hear about your cryo-EM goals and discuss how we can accelerate them.

  • No infrastructure needed

  • Streamlined onboarding & legal support

  • Highest security

Accelerate scientific discovery with our cloud-native cryo-EM platform, from data collection to high-resolution structure determination.

Subscribe to our newsletter

© 2021-2026 CryoCloud B.V. All rights reserved.

Accelerate scientific discovery with our cloud-native cryo-EM platform, from data collection to high-resolution structure determination.

Subscribe to our newsletter

© 2021-2026 CryoCloud B.V. All rights reserved.

Accelerate scientific discovery with our cloud-native cryo-EM platform, from data collection to high-resolution structure determination.

Subscribe to our newsletter

© 2021-2026 CryoCloud B.V. All rights reserved.