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Post-processing
Stage
4
of 8
Included on all plans
CryoCloud Polishing
Motion refined for each particle individually on GPU, recovering high-resolution signal that was missed during patch-based motion correction.
5x faster than Relion
Per Particle
One trajectory per particle, not per patch
What it does
Beam-induced motion is not uniform across a micrograph, and it is not uniform within a patch either. Each particle moves slightly differently from its neighbours, and that residual motion blurs the frequencies and lowers the resolution.
Polishing refines the motion of every particle individually and re-extracts it from the movie frames along its own trajectory to recover high-resolution signal for the averaged particle stack.
How it works
CryoCloud Polishing starts from your refined 3D map and particle positions. It estimates a separate motion trajectory for each particle across the frames of its original movie. A training step first learns how smooth those trajectories should be and how much each frame contributes at each resolution, using a subset of your own particles. It then re-extracts every particle with its motion corrected and its frames weighted by that model, ready for a final refinement round.
Re-extracted particles go back into refinement, which is why polishing and refinement are usually run in sequence.
Left: every particle in a patch inherits that patch's motion. Right, each particle carries its own refined trajectory.
Where it sits
This is the fine-scale version of what CryoCloud MotionCorr does at the start. MotionCorr corrects patches of a micrograph before anything is known about the particles; polishing corrects each particle once everything is.
It is a late-stage tool. Run it when a map is already good and you are trying to reach the resolution the data can support.
At a glance
Stage
Post-processing
Runs
On request, after refinement
Hardware
GPU
Inputs
Refined particles and the original movies
Outputs
Re-extracted particles with refined per-particle motion
Fed back into refinement, typically for another round.
Replaces
Relions Polishing
Availability
Included
Push a finished map towards the resolution the data can support.
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