All algorithms

Masking

Stage

3

of 8

Included on all plans

Interactive Masking

Draw a mask around the region you care about, directly on your map in the browser.

Inside your browser

Draw your region of interest on any 3D map, without download it or using external software.

Direct feedback

Immadiately see the resulting map and adjust the region, or the masks dilation and smoothing.

What it does

Interactive Masking lets you build a focused mask for local refinement, signal subtraction or focused classification without leaving CryoCloud. You outline the domain you want on the map itself, so the mask covers exactly that region. You don’t need to export the map, open ChimeraX or another external tool, or bring a mask file back in. The mask is saved to your project and is ready to use in the next job.

How it works

Open your map in the CryoCloud viewer and draw around the region of interest with the lasso tool. Draw in a single plane, or in multiple orthogonal planes if you need a more accurate mask. CryoCloud combines the outlines into a 3D volume and turns it into a mask aligned with your map. You can check the result against the density before you use it downstream.

A region drawn by hand in two orthogonal planes to create a focused mask

Where it sits

Interactive Masking comes after a consensus 3D refinement, once you have a map good enough to see the region you want. Its output goes into focused jobs downstream: local refinement, focused 3D classification and signal subtraction. AutoMask Job makes a whole-map mask automatically. Interactive Masking is the option for when you want a mask around one part of the map.

At a glance

Stage

Masking

Runs

On request, in the browser

Hardware

Runs inside the CryoCloud application

Inputs

A 3D map and your drawn region

Outputs

A focused mask

Used for focused classification and focused refinement.

Replaces

ChimeraX's Volume Eraser, CryomaskR

Availability

Included

Ask a question about one region, and mask exactly that region.

Frequently Asked Questions

If it's not covered here, please reach out to us.
…or start your free trial and see for yourself.

Is there a free trial?

Yes — when you sign up, you'll get instant access to a 30-day free trial with full access to the CryoCloud web application. You'll receive 20 hours of compute time and 2 TB of storage. No setup or infrastructure needed. After the trial, you can upgrade to a paid subscription or contact us for custom plans — no interruption to your data.

How can I start using CryoCloud?

Either go directly to out signup page or simply send us an email to hi@cryocloud.io so we can invite you to our application. You can find more information on how to get started here.

Do I need to install anything?

No - you really just need a (simple) computer, internet access and an internet browser. We took care of everything else. Read more on it here

How does your risk-free trial work?

If you are unsatisfied, you can cancel your plan within 30 days and we will refund you at 100%. You can deactivate the subscription from your account settings.

Frequently Asked Questions

If it's not covered here, please reach out to us.
…or start your free trial and see for yourself.

Is there a free trial?

Yes — when you sign up, you'll get instant access to a 30-day free trial with full access to the CryoCloud web application. You'll receive 20 hours of compute time and 2 TB of storage. No setup or infrastructure needed. After the trial, you can upgrade to a paid subscription or contact us for custom plans — no interruption to your data.

How can I start using CryoCloud?

Either go directly to out signup page or simply send us an email to hi@cryocloud.io so we can invite you to our application. You can find more information on how to get started here.

Do I need to install anything?

No - you really just need a (simple) computer, internet access and an internet browser. We took care of everything else. Read more on it here

How does your risk-free trial work?

If you are unsatisfied, you can cancel your plan within 30 days and we will refund you at 100%. You can deactivate the subscription from your account settings.

Frequently Asked Questions

If it's not covered here, please reach out to us.
…or start your free trial and see for yourself.

Is there a free trial?

Yes — when you sign up, you'll get instant access to a 30-day free trial with full access to the CryoCloud web application. You'll receive 20 hours of compute time and 2 TB of storage. No setup or infrastructure needed. After the trial, you can upgrade to a paid subscription or contact us for custom plans — no interruption to your data.

How can I start using CryoCloud?

Either go directly to out signup page or simply send us an email to hi@cryocloud.io so we can invite you to our application. You can find more information on how to get started here.

Do I need to install anything?

No - you really just need a (simple) computer, internet access and an internet browser. We took care of everything else. Read more on it here

How does your risk-free trial work?

If you are unsatisfied, you can cancel your plan within 30 days and we will refund you at 100%. You can deactivate the subscription from your account settings.

Ready to skip the queue?
Start analyzing your cryo-EM data today.

We would love hear about your cryo-EM goals and discuss how we can accelerate them.

  • No infrastructure needed

  • Streamlined onboarding & legal support

  • Highest security

Ready to skip the queue?
Start analyzing your cryo-EM data today.

We would love hear about your cryo-EM goals and discuss how we can accelerate them.

  • No infrastructure needed

  • Streamlined onboarding & legal support

  • Highest security

Ready to skip the queue?
Start analyzing your cryo-EM data today.

We would love hear about your cryo-EM goals and discuss how we can accelerate them.

  • No infrastructure needed

  • Streamlined onboarding & legal support

  • Highest security

Accelerate scientific discovery with our cloud-native cryo-EM platform, from data collection to high-resolution structure determination.

Subscribe to our newsletter

© 2021-2026 CryoCloud B.V. All rights reserved.

Accelerate scientific discovery with our cloud-native cryo-EM platform, from data collection to high-resolution structure determination.

Subscribe to our newsletter

© 2021-2026 CryoCloud B.V. All rights reserved.

Accelerate scientific discovery with our cloud-native cryo-EM platform, from data collection to high-resolution structure determination.

Subscribe to our newsletter

© 2021-2026 CryoCloud B.V. All rights reserved.