All algorithms
Masking
Stage
3
of 8
Included on all plans
Interactive Masking
Draw a mask around the region you care about, directly on your map in the browser.
Inside your browser
Draw your region of interest on any 3D map, without download it or using external software.
Direct feedback
Immadiately see the resulting map and adjust the region, or the masks dilation and smoothing.
What it does
Interactive Masking lets you build a focused mask for local refinement, signal subtraction or focused classification without leaving CryoCloud. You outline the domain you want on the map itself, so the mask covers exactly that region. You don’t need to export the map, open ChimeraX or another external tool, or bring a mask file back in. The mask is saved to your project and is ready to use in the next job.
How it works
Open your map in the CryoCloud viewer and draw around the region of interest with the lasso tool. Draw in a single plane, or in multiple orthogonal planes if you need a more accurate mask. CryoCloud combines the outlines into a 3D volume and turns it into a mask aligned with your map. You can check the result against the density before you use it downstream.

Where it sits
Interactive Masking comes after a consensus 3D refinement, once you have a map good enough to see the region you want. Its output goes into focused jobs downstream: local refinement, focused 3D classification and signal subtraction. AutoMask Job makes a whole-map mask automatically. Interactive Masking is the option for when you want a mask around one part of the map.
At a glance
Stage
Masking
Runs
On request, in the browser
Hardware
Runs inside the CryoCloud application
Inputs
A 3D map and your drawn region
Outputs
A focused mask
Used for focused classification and focused refinement.
Replaces
ChimeraX's Volume Eraser, CryomaskR
Availability
Included
Ask a question about one region, and mask exactly that region.
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