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Post-processing
Stage
4
of 8
Included on all plans
Automated Map Segmentation
Segment your map into multiple bodies based on the continuity of your map.
Automatic
ONly requires you to specify the number of subunits
Automatic
No regions traced by hand
What it does
The automated map segmentation separates the body of your provided maps into multiple subunits based on the specfied number of regions. It will also automatically crate a region mask encompassing the subunits if turned on.
How it works
Segmentation divides the density into regions, one per subunit, along the boundaries where one component meets the next. It also applies smoothing filters to automatically detect boundaries where one protein or subunit ends and another begins.
The output is one region per subunit, which can be used to model each component separately, to focus on one part of the complex, or to check that the map's composition matches what you expected.
Schematic, not measured data. Given the number of subunits, the map on the left is divided into one region per subunit on the right.
Where it sits
Automated segmentation can be run on top of any job that creates 3D density map such as the Initial model job, 3D classification and 3D Refinement.
At a glance
Stage
Post-processing
Runs
On request, after refinement
Hardware
CPUs
Inputs
A map and the number of subunits
Outputs
One region per subunit
Ready for model building or for focusing on one component.
Replaces
Chimera Segger
Availability
Included
Go from one map to its subunits without tracing a boundary.
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